
Animal feeding stuffs - Determination of amino acids content
出版:International Organization for Standardization

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ISO 13903:2005 describes the determination of free (synthetic and natural) and total (peptide-bound and free) amino acids in feeding stuffs, using an amino acid analyser or HPLC equipment. It is applicable to the following amino acids: sum of cystine and cysteine; methionine; lysine; threonine; alanine; arginine; aspartic acid; glutamic acid; glycine; histidine; isoleucine; leucine; phenylalanine; proline; serine; tyrosine; valine.The method does not distinguish between the salts of amino acids, nor does it differentiate between D and L forms of amino acids. It is not valid for the determination of tryptophan or hydroxy analogues of amino acids.Limits of quantification depend on the chromatographic equipment, but levels as low as: 0,3 g/kg total lysine; 0,25 g/kg total methionine; 0,35 g/kg total cystine plus cysteine; 0,2 g/kg total threonine; 0,035g/kg free lysine; 0,035g/kg free methionine; and 0,03g/kg free threonine can typically be analysed.
UNE EN ISO 13903:2006 - Identical
NS EN ISO 13903 Ed. 1 (2005) - Identical
UNI EN ISO 13903:2005 - Identical
DIN EN ISO 13903 (2005-09) - Identical
BS EN ISO 13903:2005 - Identical
SN EN ISO 13903:2005 - Identical
JIS K 1359:1985 - Equivalent
NBN EN ISO 13903:2005 - Identical
NF EN ISO 13903:2005 - Identical
I.S. EN ISO 13903:2005 - Identical
SS EN ISO 13903 Ed. 1 (2005) - Identical
PN EN ISO 13903:2006 - Identical
NEN EN ISO 13903:2005 - Identical